Coding

Part:BBa_K1998001:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-13)


YCF54 - ChlM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 912
    Illegal BamHI site found at 316
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 478


Design Notes

Codon optimisation for bacteria, order of parts in operon, illegal enzyme kinetics

Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References